Journal: Clinical and Experimental Obstetrics & Gynecology

Article Title: Predicting Tumour Progression of ID-8 Syngeneic Mouse Ovarian Cancer with Blood Biomarkers of CA-125, IL-6 and VEGF: A Prospective Laboratory-Based Study

doi: 10.31083/j.ceog5010212

Figure Lengend Snippet: Fig. 5. Levels of biomarkers in blood and ascitic fluids of mice with tumour. Levels of CA-125 (A), IL-6 (B), VEGF (C) and ascitic

Article Snippet: Mouse IL-6 DuoSet and mouse VEGF DuoSet were purchased from R&D systems (Minneapolis, MN, USA).

Techniques:

Journal: Journal of Applied Physiology

Article Title: Skeletal myofiber VEGF deficiency leads to mitochondrial, structural, and contractile alterations in mouse diaphragm

doi: 10.1152/japplphysiol.00779.2018

Figure Lengend Snippet: Diaphragm contractile function in skeletal muscle-specific vascular endothelial growth factor (VEGF)-deficient (SkmVEGF−/−) mice compared with wild-type (WT) controls. Isolated diaphragm fiber bundles were stimulated at increasing stimulation frequencies to measure isometric specific force (A) and normalized relative to maximum force (B), with fatigability assessed during repeated stimulations (C). Data are means ± SD; *P < 0.01; §P < 0.05 SkmVEGF−/− vs. WT.

Article Snippet: VEGF protein in diaphragm tissue was quantified by ELISA and normalized to total protein, in accordance with the manufacturer’s instructions (VEGF Mouse ELISA, R&D Systems, La Jolla, CA).

Techniques: Isolation

Journal: Journal of Applied Physiology

Article Title: Skeletal myofiber VEGF deficiency leads to mitochondrial, structural, and contractile alterations in mouse diaphragm

doi: 10.1152/japplphysiol.00779.2018

Figure Lengend Snippet: Diaphragm structural properties in skeletal muscle-specific vascular endothelial growth factor (VEGF)-deficient (SkmVEGF−/−) mice compared with wild-type (WT) controls, which included assessment of capillary-to-fiber (C/F) ratio (A), capillary density (CD) (B), and mean fiber cross-sectional area (FCSA) (C) (n = 5 per group). Representative immunofluorescent diaphragm sections from WT (D) and SkmVEGF−/− (E) mice (fiber boundaries stained red, type I fibers green, and type II fibers unstained), revealed no change in fiber type proportions (F) but a type I specific fiber atrophy (G) in SkmVEGF−/− mice compared with WT controls (n = 8–11 per group). Data are presented as means ± SD; *P < 0.05 SkmVEGF−/− vs. WT.

Article Snippet: VEGF protein in diaphragm tissue was quantified by ELISA and normalized to total protein, in accordance with the manufacturer’s instructions (VEGF Mouse ELISA, R&D Systems, La Jolla, CA).

Techniques: Staining

Journal: Journal of Applied Physiology

Article Title: Skeletal myofiber VEGF deficiency leads to mitochondrial, structural, and contractile alterations in mouse diaphragm

doi: 10.1152/japplphysiol.00779.2018

Figure Lengend Snippet: Protein expression in the diaphragm of the key contractile proteins myosin heavy chain (MyHC) (A) and sarcomeric actin (B) as well as respective representative blots (C) in skeletal muscle-specific vascular endothelial growth factor (VEGF)-deficient (SkmVEGF−/−) mice compared with wild-type (WT) controls. Data were normalized to the loading control GAPDH and calculated as fold change (∆) vs. WT. Data are presented as means ± SD; *P = 0.01 SkmVEGF−/− vs. WT.

Article Snippet: VEGF protein in diaphragm tissue was quantified by ELISA and normalized to total protein, in accordance with the manufacturer’s instructions (VEGF Mouse ELISA, R&D Systems, La Jolla, CA).

Techniques: Expressing, Control

Journal: Journal of Applied Physiology

Article Title: Skeletal myofiber VEGF deficiency leads to mitochondrial, structural, and contractile alterations in mouse diaphragm

doi: 10.1152/japplphysiol.00779.2018

Figure Lengend Snippet: Diaphragm protein expression along with representative blots of regulatory muscle signaling proteins measured in skeletal muscle-specific vascular endothelial growth factor (VEGF)-deficient (SkmVEGF−/−) mice compared with wild-type (WT) controls, which included the key atrogene muscle atrophy F-box (MAFbx) (A) and reactive oxygen species (ROS) source NADPH oxidase (subunit gp91phox) (B), as well as the upstream VEGF activators hypoxia inducible factor (HIF)-1α (C) and PGC-1α (D). Data were normalized to the loading control GAPDH and calculated as fold change (∆) vs. WT. Data are presented as means ± SD.

Article Snippet: VEGF protein in diaphragm tissue was quantified by ELISA and normalized to total protein, in accordance with the manufacturer’s instructions (VEGF Mouse ELISA, R&D Systems, La Jolla, CA).

Techniques: Expressing, Control

Journal: Journal of Applied Physiology

Article Title: Skeletal myofiber VEGF deficiency leads to mitochondrial, structural, and contractile alterations in mouse diaphragm

doi: 10.1152/japplphysiol.00779.2018

Figure Lengend Snippet: Diaphragm mitochondrial respiratory function and reactive oxygen species (ROS) production. Rate of oxygen consumption (JO2) measured during a high-resolution respirometry substrate-uncoupler-inhibitor-titration (SUIT) protocol. A: LEAK: glutamate+malate for LEAK state respiration. OXPHOS_CI: ADP for the phosphorylating state with substrates provided to complex I. OXPHOS_CI+II: ADP+succinate. ETS: Carbonyl cyanide m-chlorophenyl hydrazine for uncoupled respiration and electron transport system capacity. ETS_CII: Rotenone added to inhibit complex I. B: H2O2 flux measured simultaneous to JO2. Main effect of genotype present (F[1,75] = 14.57, P = 0.0003); *different to wild-type (WT). C: OXPHOS coupling efficiency was calculated as (1-LEAK/OXPHOS_CI+II). D: Flux control ratio (FCR) for OXPHOS_CI was calculated as (OXPHOS_CI/ETS). E: FCR for OXPHOS_CI+II was calculated as (OXPHOS_CI+II/ETS). F: Substrate control ratio (SCR) for succinate was calculated as (OXPHOS_CI+II/OXPHOS_CI). Error bars are SD; n = 8–10. SkmVEGF, skeletal myofiber vascular endothelial growth factor.

Article Snippet: VEGF protein in diaphragm tissue was quantified by ELISA and normalized to total protein, in accordance with the manufacturer’s instructions (VEGF Mouse ELISA, R&D Systems, La Jolla, CA).

Techniques: Titration, Control